Biological Fermenter

Bioreactor | Fully Automatic Aseptic Microbial Culture Bioreactor

💡Biological fermentation tanks, also known as bioreactors, are made of 316L food-grade stainless steel. They support SIP (Sterilization-in-Place) aseptic sterilization, CIP (Cleaning-in-Place) automated cleaning, and high-precision PID parameter control, and are suitable for the fully automated aseptic production of microbial fermentation products, cell cultures, enzyme preparations, antibiotics, and probiotics.

I. Product Introduction

Biological fermentation tanks (bioreactors) are core aseptic reaction vessels used for the cultivation of microorganisms (bacteria, yeast, fungi) or animal and plant cells. They integrate a full suite of functions, including sterilization, aeration and agitation, temperature and humidity control, pH adjustment, dissolved oxygen control, automatic defoaming, and intelligent feed addition, and operate under aseptic, sealed conditions throughout the entire process. They are widely used in large-scale biological fermentation production across industries such as biopharmaceuticals, food fermentation, enzyme preparation, amino acid synthesis, probiotic cultivation, and biofuels.

II. How It Works

Pour the prepared culture medium into the fermenter, perform full sterilization using the built-in SIP steam system, and inoculate with microbial strains or animal and plant cells. The equipment precisely controls the temperature (25–45°C) via a jacket heat exchanger and built-in coils, and is equipped with a stirring system and a microporous gas distributor to enhance the transfer of oxygen from sterile air. The system automatically adjusts acidity and alkalinity to stabilize the pH and intelligently adds defoaming agents to prevent foam overflow. During the production process, carbon sources, nitrogen sources, and precursor materials are automatically replenished according to the metabolic requirements of the microbial strain. The entire process is intelligently controlled by a PLC. Upon completion of fermentation, discharge is performed under sealed conditions, ensuring a sterile, stable, and highly active fermentation environment throughout the entire process.

III. Main Structural Components

✅ Stainless steel tank (SUS316L, electropolished inner surface)

Pharmaceutical-grade 316L stainless steel with mirror-finish electropolished inner walls; no dead corners or residue; corrosion-resistant and easy to sanitize; meets aseptic production standards.

✅ Agitation System (Mechanical Agitation / Magnetic Agitation + Variable-Frequency Motor)

Variable-frequency speed-controlled stirring, with an optional seal-less magnetic stirring system to prevent bacterial contamination and accommodate the oxygenation requirements of different microbial strains.

✅ Gas Distributor (Annular/Microporous)

It evenly disperses sterile air, significantly increasing the gas-liquid contact area and enhancing oxygen transfer efficiency.

✅ Heat-exchange jacket/built-in heat-exchange coil

Rapid heating and cooling, precise maintenance of a constant temperature environment for fermentation, tailored to the metabolic requirements of specific microbial strains.

✅ Online Smart Sensors

Real-time monitoring of temperature, pH, dissolved oxygen (DO), foam, and CO₂ concentration, with accurate and traceable data.

✅ Precision Peristaltic Pump Assembly

Automatic replenishment of feedstock, addition of acid, addition of alkali, and addition of defoaming agent, with precise micro-dosing.

✅ High-Efficiency Air Filtration System

0.01 μm hydrophobic membrane filtration intercepts bacteria and particles in the air, ensuring a sterile air supply.

✅ SIP Steam Sterilization System

Fully automatic in-place sterilization; no need to disassemble the unit; the entire process is performed in a sterile, sealed environment.

✅ CIP Automatic Cleaning Ball

360° spray cleaning with no blind spots, compliant with GMP cleanroom cleaning standards.

✅ PLC Touchscreen Control System

Visual operation interface with built-in historical graphs, data storage, and parameter logging, ensuring full traceability of production data.

IV. Core Advantages

✅ High-level sterility assurance to prevent contamination

Supports fully automated SIP (Sterilization-in-Place) with a fully sealed interface design. Combined with a sterile air filtration system, this eliminates microbial contamination at the source and ensures the yield of qualified fermentation products.

✅ Intelligent control of high-precision parameters

Using a PID intelligent algorithm, the system achieves a temperature control accuracy of ±0.1°C, a pH accuracy of ±0.02, and a dissolved oxygen (DO) accuracy of ±21 TP3T, precisely meeting the growth and metabolic needs of various bacterial strains.

✅ Extremely high oxygen transfer efficiency, high fermentation yield

With an optimized agitator design and a microporous aeration system, the oxygen transfer coefficient (KLa) can reach 200–1000 h⁻¹, resulting in high microbial activity and high fermentation yield.

✅ Modular expansion, compatible with multiple processes

It offers flexible expansion capabilities for exhaust gas analysis, metabolic monitoring, and external circulation systems, making it suitable for all scenarios—from small-scale laboratory trials and pilot-scale testing to industrial-scale mass production.

✅ GMP-compliant standardized design

The materials used in the equipment are traceable, and a complete set of DQ/IQ/OQ/PQ validation documents is available, ensuring full compliance with GMP production standards for biopharmaceuticals and food fermentation.

V. Typical Use Cases

✅ Antibiotic Fermentation

Suitable for the microbial fermentation production of antimicrobial drugs such as penicillin, erythromycin, and cephalosporins.

✅ Enzyme Preparation Production

Used for the large-scale cultivation and production of industrial enzyme preparations such as proteases, amylases, and cellulases.

✅ Amino Acid Fermentation

Supports fermentation processes for food-grade and pharmaceutical-grade amino acids such as glutamic acid and lysine.

✅ Probiotic Cultivation

Suitable for the sterile cultivation and proliferation of beneficial microorganisms such as lactic acid bacteria, bifidobacteria, and yeast.

✅ Vaccine Cell Culture

Used for the aseptic culture of medical cells such as CHO cells and Vero cells.

✅ Biofuel Fermentation

Suitable for fermentation and concentration processes used in the production of biofuels such as ethanol and butanol.

VI. Optional Configurations

✅ Magnetic stirring (no mechanical seal)

No sealing dead spots, zero risk of contamination, and suitable for high-purity, sterile production.

✅ Automatic Online Sampling System

Sealed, sterile sampling prevents contamination from manual sampling and ensures the authenticity of the samples.

✅ Feed Tank for Fermentation

Equipped with sterile feed storage, it enables continuous, precise feed production.

VII. Key Technical Specifications

VIII. Service Commitment

We provide end-to-end services ranging from process adaptation of microbial strains, equipment selection, customized solutions, manufacturing, on-site installation and commissioning, coordination of GMP validation documentation, to operator training. Our equipment supports custom-sized tanks and tailored process parameters, with nationwide delivery and installation available, as well as a 24-hour rapid on-site after-sales response in Chongqing.

🔗 Recommended Compatible Devices

Complete Turnkey Solutions for Bioreactor Fermentation Lines:
✅ Complete aseptic production line:Support Systems
✅ Preparation and Storage of Materials:Mixing and Storage Tank Equipment
✅ Post-fermentation treatment:Separation and Purification Equipment